A movie of a vesicle fusion detected by the algorithm.
Color code:
[ Purple] Static Vesicle.
[ Yellow ] Fusing vesicle.
[ Red] Moving vesicle.
[Cyan] Short lived detection (No decision about type of dynamics can be made with a short time series).
Detections made by the algorithm in VAMP2-GFP7.1 cells. The GFP fluorescent protein is pH dependent. Thus, the proteins fluoresce only when a vesicle fuses to the cell membrane and a pore that connects vesicle lumen and exterior is open. Notice the difference with the second cell. It this case, the cell is treated with a toxin that changes the pH of the vesicle lumen. The GFP proteins fluoresce before vesicle fusion.